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Figure 1 | Arthritis Research & Therapy

Figure 1

From: Prostaglandin PGE2 at very low concentrations suppresses collagen cleavage in cultured human osteoarthritic articular cartilage: this involves a decrease in expression of proinflammatory genes, collagenases and COL10A1, a gene linked to chondrocyte hypertrophy

Figure 1

Inhibition of collagen cleavage by prostaglandin E2 (PGE2) and transforming growth factor (TGF)-β2. (a–d) The effect of 5 ng/ml TGF-β2 and PGE2 at various concentrations (1 pg to 10 ng/ml) on collagen cleavage in human osteoarthritic (OA) explants from patients of indicated age and gender; (e) means ± SDs for all four patients; (f) inhibition of type II collagen cleavage by collagenase in human OA articular explants by 10 pg/ml PGE2; (g) concentration relationship between PGE2 in control cultures and collagen cleavage. The average medium levels of PGE2 in the dose-dependent studies were as follows: 6.3 ± 1.2 pg/ml for the 77-year-old female (a); 13.9 ± 4.1 pg/ml for the 50-year-old female (b); 7.8 ± 2.1 pg/ml for the 66-year-old male (c); and 61.6 ± 7.6 pg/ml for the 82-year-old female (d). (f) Thirteen OA articular cartilage explants were cultured with (10 pg/ml PGE2) or without (control) for 16 days and collagen cleavage was evaluated by the accumulation of C1,2C neoepitope in the medium and chymotrypsin-derived cartilage extracts. (g) The relationship between PGE2 concentration and collagen cleavage in 16 OA explants that served as controls. Significant differences from the control (P < 0.05) are indicated by asterisks.

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