Figure 4

TauCl inhibited NF-κB binding activity. Synovial cells (2 × 10⁶ cells/100-mm dish/5-ml serum-free media) were pretreated with taurine chloramine (TauCl) or taurine (Tau) 30 min prior to IL-1β stimulation for 90 min. Nuclear extracts were prepared for electrophoretic mobility shift assay (EMSA). IL-1β stimulation increased nuclear levels of nuclear factor-κB (NF-κB). At a concentration of 800 μmol/l, TauCl completely inhibited NF-κB binding. Antibodies against the p65 subunit of NF-κB induced a gel shift in the NF-κB band. Three independent experiments were performed with cells from two patients.