Figure 5

TauCl inhibited the migration of NF-κB into the nucleus. To visualize the translocation of nuclear factor-κB (NF-κB), synovial cells (4 × 10⁴ cells/well in four-well Lab-Tek chamber slides) were cultured. After serum starvation overnight, the cells were treated with taurine chloramine (TauCl) 30 min before stimulation with IL-1β (10 ng/ml) for 90 min. IL-1β stimulation induced the migration of NF-κB from the cytoplasm into the nucleus (second column), whereas NF-κB was found only in the cytoplasm of nonstimulated cells (first column). At a concentration of 800 μmol/l, TauCl completely inhibited the migration of NF-κB into the nucleus (fifth column). All pictures were taken at a magnification of 200×. Three independent experiments were performed in duplicate with cells from two patients.