Table 1 Primer and probe sequences of desired genes
From: Passage and reversal effects on gene expression of bovine meniscal fibrochondrocytes
Target gene (GenBank accession number, product size) | Forward primer (5'→3') | Reverse primer (5'→3') | Probe (5'→3') |
|---|---|---|---|
Collagen-I (NM-174520, 97 bp) | CATTAGGGGTCACAATGGTC | TGGAGTTCCATTTTCACCAG | ATGGATTTGAAGGGACAGCCTTGG |
Collagen-IIa (X02420, 76 bp) | AACGGTGGCTTCCACTTC | GCAGGAAGGTCATCTGGA | ATGACAACCTGGCTCCCAACACC |
Aggrecan (U76615, 76 bp) | GCTACCCTGACCCTTCATC | AAGCTTTCTGGGATGTCCAC | TGACGCCATCTGCTACACAGGTGA |
COMP (X74326, 72 bp) | TCAGAAGAGCAACGCAGAC | TCTTGGTCGCTGTCACAA | CAGAGGGATGTGGACCACGACTTC |
GAPDH (U85042, 86 bp) | ACCCTCAAGATTGTCAGCAA | ACGATGCCAAAGTGGTCA | CCTCCTGCACCACCAACTGCTT |