The influence of the NOD Nss1/Idd5 loci on sialadenitis and gene expression in salivary glands of congenic mice

Table 1 Validation of microarray by QPCR

Gene symbol	B10.Q versus B10.Q.Nss1			B10.Q versus B10.Q.Nss1/Idd5			B10.Q.Nss1 versus B10.Q.Nss1/Idd5
	Array FC	QPCR FC	QPCR P value	Array FC	QPCR FC	QPCR P value	Array FC	QPCR FC	QPCR P value
Ltb	2	2.4	0.03	2.7	4.3	0.01	1.3	1.8	0.25
Egf	-3	-5.9	0.04	-1.9	-1.6	0.17	1.6	3.5	0.01
Ccl19	2.7	2.5	< 0.01	3.3	8.6	0.01	1.2	3.5	0.17
Klk9	-2.2	-5.0	0.03	-1.4	-1.3	0.44	1.5	3.8	< 0.01
Stat1	2.2	1.3	0.3	1.8	1.6	0.03	-1.3	1.3	0.28
Zap70	2.8	2.5	< 0.01	2.4	3.6	0.02	1.1	1.5	0.34
Cd19	3.1	5.4	0.02	3.5	20.9	0.04	1.2	3.9	0.25
Sell	-	-	-	3	9.2	0.09	2.7	3.7	0.25
Dock7	-1.6	-1.9	< 0.01	-1.8	-1.6	< 0.01	-1.1	1.2	0.2
Fas	2.3	1.2	0.34	2	1.5	0.01	-	-	-

The three columns under each comparison (gene list) represent, from left to right, the fold change (FC) from the microarray gene list, the FC for the quantitative reverse transcriptase PCR (QPCR) and the statistical significance from the QPCR (P value). Genes with positive FC were upregulated in the congenic mice compared with B10.Q, and upregulated in B10.Q.Nss1/Idd5 when compared with B10.Q.Nss1. The genes Ltb, Egf, Ccl19, Klk9 and Stat1 were selected from the gene list of the standard analysis, and Zap70, Cd19, Sell, Dock7 and Fas were selected from the gene list of the alternative analysis.

ISSN: 1478-6362