Table 3 Differentially expressed genes in OA bone with roles in osteogenesis, angiogenesis and chondrogenesis
Real-time PCR | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Rank | GenBank | Role | Cell type | Pathway | Symbol | Name | t OA/CTL | t OA/OP | Symbol | OA/CTL |
ADAMTS4 | 0.11 | |||||||||
2 | NM_001124 | A, B, C | OB, OC, OS, CB | TGF-β/BMP | ADM | Adrenomedullin | -12.620 | -7.904 | ADM | 0.09 |
3 | NM_002450 | B | OB | MT1L | Metallothionein 1L | -8.342 | -6.843 | |||
4 | NM_016109 | A | WNT | ANGPTL4 | Angiopoietin-like 4 | -6.165 | -8.052 | |||
5 | NM_015675 | B | OB, OC, CB | TGF-β/BMP | GADD45B | Growth arrest and DNA-damage-inducible, beta | -8.102 | -5.985 | GADD45B | 0.15 |
7 | NM_000358 | A, B | TGF-β/BMP | TGF-BI | Transforming growth factor, beta-induced | -6.915 | -5.741 | |||
9 | NM_017817 | RAB20 | RAB20, member RAS oncogene family | -5.147 | -6.982 | |||||
10 | NM_004967 | B, C | OB, CB | WNT & TGF-β/BMP | IBSP | Integrin-binding sialoprotein | -5.088 | -5.865 | IBSP | 0.25 |
12 | AB014526 | B | OB | MFAP3L | Microfibrillar-associated protein 3-like | 5.284 | 4.983 | |||
13 | NM_004142 | A, B, C | OB, OC, CB | WNT | MMP25 | Matrix metalloproteinase 25 | 4.942 | 7.183 | MMP25 | 2.60 |
15 | NM_002025 | B | TGF-β/BMP | FMR2 | Fragile × mental retardation 2 | 4.915 | 4.851 | |||
18 | NM_005953 | B | OB | MT2A | Metallothionein 2A | -4.638 | -7.071 | MT2A | 0.18 | |
20 | NM_013258 | A, B | M OC | PYCARD | PYD and CARD domain containing | 6.567 | 4.355 | |||
21 | NM_014467 | SRPX2 | Sushi-repeat-containing protein, X-linked 2 | -4.333 | -4.918 | |||||
22 | NM_003155 | A, B, C | OB, OC, CB | STC1 | Stanniocalcin 1 | -6.656 | -4.274 | STC1 | 0.16 | |
23 | NM_001109 | B | OC | ADAM8 | A disintegrin and metalloproteinase domain 8 | 5.426 | 4.269 | |||
24 | NM_020203 | B | OB, OS | TGF-β/BMP | MEPE | Matrix, extracellular phosphoglycoprotein with ASARM motif | -5.036 | -4.263 | MEPE | 0.11 |
28 | S68954 | B | OB | MT1G | Metallothionein 1G | -4.202 | -7.630 | |||
29 | AK026438 | B | OB | GALNT4 | Polypeptide N-acetylgalactosaminyltransferase 4 | 5.259 | 4.195 | |||
30 | NM_000474 | B | OB | WNT | TWIST1 | Twist homolog 1 | -4.194 | -4.910 | TWIST1 | 0.31 |
33 | NM_000655 | B | M | SELL | Selectin L | 4.852 | 4.179 | |||
36 | NM_002341 | A, B, C | OB, CB | LTB | Lymphotoxin beta | 4.465 | 4.086 | |||
37 | NM_003226 | A | TFF3 | Trefoil factor 3 | 4.080 | 4.074 | ||||
42 | NM_000607 | B | M | ORM1 | Orosomucoid 1 | 4.284 | 3.982 | |||
46 | NM_005542 | INSIG1 | Insulin induced gene 1 | -7.756 | -3.878 | INSIG1 | 0.55 | |||
49 | NM_001302 | B | M | CORT | Cortistatin | -6.713 | -3.843 | |||
52 | NM_000598 | A, B, C | OB, OC, CB | WNT & TGF-β/BMP | IGFBP3 | Insulin-like growth factor binding protein 3 | -3.772 | -3.893 | ||
54 | NM_012479 | A, B | OB | YWHAG | Tyrosine 3-/tryptophan 5-monooxygenase activation protein, gamma | -3.759 | -5.355 | |||
59 | NM_014624 | B | OB | WNT | S100A6 | S100 calcium binding protein A6 | 8.214 | 3.710 | ||
61 | NM_006732 | A, B | OB | FOSB | FBJ murine osteosarcoma viral oncogene homolog B | 3.682 | 3.926 | |||
64 | NM_002961 | A, B | OB | WNT | S100A4 | S100 calcium binding protein A4 | 4.407 | 3.596 | S100A4 | 2.43 |
65 | NM_016184 | B | M | CLECSF6 | C-type lectin domain family 4, member A | 4.575 | 3.594 | |||
68 | NM_006184 | B | NUCB1 | Nucleobindin 1 | -3.551 | -5.513 | ||||
69 | NM_001300 | A | WNT & TGF-β/BMP | KLF6 | Kruppel-like factor 6 | -4.047 | -3.544 | KLF6 | 0.41 | |
70 | NM_004864 | A, B | OB, CB | TGF-β/BMP | GDF15 | Growth differentiation factor 15 | -3.541 | -4.269 | ||
73 | U79271 | B | OB, OC | WNT | AKT3 | V-akt murine thymoma viral oncogene homolog 3 | 3.507 | 4.876 | ||
76 | S83282 | A, B | OB, OC | MIF | Macrophage migration inhibitory factor | -3.654 | -3.496 | |||
81 | NM_005213 | B | OC | CSTA | Cystatin A | 4.450 | 3.447 | |||
82 | NM_000647 | A, B | M OC | CCR2 | Chemokine (C-C motif) receptor 2 | 7.277 | 3.446 | |||
85 | NM_001911 | A | CTSG | Cathepsin G | 8.474 | 3.411 | ||||
87 | NM_001122 | WNT | ADFP | Adipose differentiation-related protein | -3.368 | -10.219 | ADFP | 0.21 | ||
92 | NM_006254 | A, B | OB | PRKCD | Protein kinase C, delta | 5.549 | 3.319 | |||
94 | NM_002162 | A | ICAM3 | Intercellular adhesion molecule 3 | 3.304 | 5.798 | ||||
95 | NM_001885 | B | OB, OC | CRYAB | Crystallin, alpha B | -3.296 | -7.616 | |||
96 | D17152 | A | WNT | SOD2 | superoxide dismutase 2 | -6.347 | -3.278 | |||
102 | NM_003256 | A | WNT | TIMP4 | Tissue inhibitor of metalloproteinase 4 | -6.319 | -3.250 | TIMP4 | 0.08 | |
105 | NM_000698 | M | ALOX5 | Arachidonate 5-lipoxygenase | 3.229 | 4.736 | ||||
106 | NM_014029 | B | OC | RAC2 | Rho family, small GTP binding protein Rac2 | 3.227 | 5.325 | |||
108 | NM_003670 | B, C | OB, CB | TGF-β/BMP | DEC1 | Differentially expressed in chondrocytes 1 | -3.203 | -3.783 | ||
109 | NM_002067 | B | OB | GNA11 | Guanine nucleotide binding protein, alpha 11 | -4.353 | -3.202 | |||
115 | AF263545 | B | OB | SLC14A1 | Solute carrier family 14, member 1 | 3.163 | 5.759 | |||
120 | NM_005127 | B | M | CLECSF2 | C-type lectin domain family 2, member B | -7.330 | -3.141 | |||
123 | NM_004252 | B | WNT | NHERF1 | Sodium/Hydrogen exchanger regulatory factor 1 | 5.280 | 3.138 | NHERF1 | 1.71 | |
124 | NM_006186 | B | OB | NR4A2 | Nuclear receptor subfamily 4, group A, member 2 | -3.128 | -3.354 | |||
133 | NM_000104 | B | OC | CYP1B1 | Cytochrome P450, family 1, subfamily B, 1 | -3.075 | -5.148 | |||
136 | NM_003005 | B | SELP | Selectin P | 8.740 | 3.066 | ||||
139 | NM_004334 | B | OC | BST1 | Bone marrow stromal cell antigen 1 (CD157) | 3.037 | 3.601 | |||
141 | AK023619 | A | CRIM1 | Cysteine-rich motor neuron 1 | -3.043 | -3.271 | ||||
143 | NM_013332 | A | WNT | HIG2 | Hypoxia-inducible protein 2 | -6.019 | -3.035 | |||
144 | NM_001845 | A, B | TGF-β/BMP | COL4A1 | Collagen, type IV, alpha 1 | -3.028 | -8.960 | COL4A1 | 0.57 | |
146 | NM_016224 | A, B | OB | WNT | SNX9 | Sorting nexin 9 | -3.025 | -6.086 | ||