Skip to main content

Advertisement

Table 3 Differentially expressed genes in OA bone with roles in osteogenesis, angiogenesis and chondrogenesis

From: Microarray gene expression profiling of osteoarthritic bone suggests altered bone remodelling, WNT and transforming growth factor-β/bone morphogenic protein signalling

          Real-time PCR
Rank GenBank Role Cell type Pathway Symbol Name t OA/CTL t OA/OP Symbol OA/CTL
          ADAMTS4 0.11
2 NM_001124 A, B, C OB, OC, OS, CB TGF-β/BMP ADM Adrenomedullin -12.620 -7.904 ADM 0.09
3 NM_002450 B OB   MT1L Metallothionein 1L -8.342 -6.843   
4 NM_016109 A   WNT ANGPTL4 Angiopoietin-like 4 -6.165 -8.052   
5 NM_015675 B OB, OC, CB TGF-β/BMP GADD45B Growth arrest and DNA-damage-inducible, beta -8.102 -5.985 GADD45B 0.15
7 NM_000358 A, B   TGF-β/BMP TGF-BI Transforming growth factor, beta-induced -6.915 -5.741   
9 NM_017817     RAB20 RAB20, member RAS oncogene family -5.147 -6.982   
10 NM_004967 B, C OB, CB WNT & TGF-β/BMP IBSP Integrin-binding sialoprotein -5.088 -5.865 IBSP 0.25
12 AB014526 B OB   MFAP3L Microfibrillar-associated protein 3-like 5.284 4.983   
13 NM_004142 A, B, C OB, OC, CB WNT MMP25 Matrix metalloproteinase 25 4.942 7.183 MMP25 2.60
15 NM_002025 B   TGF-β/BMP FMR2 Fragile × mental retardation 2 4.915 4.851   
18 NM_005953 B OB   MT2A Metallothionein 2A -4.638 -7.071 MT2A 0.18
20 NM_013258 A, B M OC   PYCARD PYD and CARD domain containing 6.567 4.355   
21 NM_014467     SRPX2 Sushi-repeat-containing protein, X-linked 2 -4.333 -4.918   
22 NM_003155 A, B, C OB, OC, CB   STC1 Stanniocalcin 1 -6.656 -4.274 STC1 0.16
23 NM_001109 B OC   ADAM8 A disintegrin and metalloproteinase domain 8 5.426 4.269   
24 NM_020203 B OB, OS TGF-β/BMP MEPE Matrix, extracellular phosphoglycoprotein with ASARM motif -5.036 -4.263 MEPE 0.11
28 S68954 B OB   MT1G Metallothionein 1G -4.202 -7.630   
29 AK026438 B OB   GALNT4 Polypeptide N-acetylgalactosaminyltransferase 4 5.259 4.195   
30 NM_000474 B OB WNT TWIST1 Twist homolog 1 -4.194 -4.910 TWIST1 0.31
33 NM_000655 B M   SELL Selectin L 4.852 4.179   
36 NM_002341 A, B, C OB, CB   LTB Lymphotoxin beta 4.465 4.086   
37 NM_003226 A    TFF3 Trefoil factor 3 4.080 4.074   
42 NM_000607 B M   ORM1 Orosomucoid 1 4.284 3.982   
46 NM_005542     INSIG1 Insulin induced gene 1 -7.756 -3.878 INSIG1 0.55
49 NM_001302 B M   CORT Cortistatin -6.713 -3.843   
52 NM_000598 A, B, C OB, OC, CB WNT & TGF-β/BMP IGFBP3 Insulin-like growth factor binding protein 3 -3.772 -3.893   
54 NM_012479 A, B OB   YWHAG Tyrosine 3-/tryptophan 5-monooxygenase activation protein, gamma -3.759 -5.355   
59 NM_014624 B OB WNT S100A6 S100 calcium binding protein A6 8.214 3.710   
61 NM_006732 A, B OB   FOSB FBJ murine osteosarcoma viral oncogene homolog B 3.682 3.926   
64 NM_002961 A, B OB WNT S100A4 S100 calcium binding protein A4 4.407 3.596 S100A4 2.43
65 NM_016184 B M   CLECSF6 C-type lectin domain family 4, member A 4.575 3.594   
68 NM_006184 B    NUCB1 Nucleobindin 1 -3.551 -5.513   
69 NM_001300 A   WNT & TGF-β/BMP KLF6 Kruppel-like factor 6 -4.047 -3.544 KLF6 0.41
70 NM_004864 A, B OB, CB TGF-β/BMP GDF15 Growth differentiation factor 15 -3.541 -4.269   
73 U79271 B OB, OC WNT AKT3 V-akt murine thymoma viral oncogene homolog 3 3.507 4.876   
76 S83282 A, B OB, OC   MIF Macrophage migration inhibitory factor -3.654 -3.496   
81 NM_005213 B OC   CSTA Cystatin A 4.450 3.447   
82 NM_000647 A, B M OC   CCR2 Chemokine (C-C motif) receptor 2 7.277 3.446   
85 NM_001911 A    CTSG Cathepsin G 8.474 3.411   
87 NM_001122    WNT ADFP Adipose differentiation-related protein -3.368 -10.219 ADFP 0.21
92 NM_006254 A, B OB   PRKCD Protein kinase C, delta 5.549 3.319   
94 NM_002162 A    ICAM3 Intercellular adhesion molecule 3 3.304 5.798   
95 NM_001885 B OB, OC   CRYAB Crystallin, alpha B -3.296 -7.616   
96 D17152 A   WNT SOD2 superoxide dismutase 2 -6.347 -3.278   
102 NM_003256 A   WNT TIMP4 Tissue inhibitor of metalloproteinase 4 -6.319 -3.250 TIMP4 0.08
105 NM_000698   M   ALOX5 Arachidonate 5-lipoxygenase 3.229 4.736   
106 NM_014029 B OC   RAC2 Rho family, small GTP binding protein Rac2 3.227 5.325   
108 NM_003670 B, C OB, CB TGF-β/BMP DEC1 Differentially expressed in chondrocytes 1 -3.203 -3.783   
109 NM_002067 B OB   GNA11 Guanine nucleotide binding protein, alpha 11 -4.353 -3.202   
115 AF263545 B OB   SLC14A1 Solute carrier family 14, member 1 3.163 5.759   
120 NM_005127 B M   CLECSF2 C-type lectin domain family 2, member B -7.330 -3.141   
123 NM_004252 B   WNT NHERF1 Sodium/Hydrogen exchanger regulatory factor 1 5.280 3.138 NHERF1 1.71
124 NM_006186 B OB   NR4A2 Nuclear receptor subfamily 4, group A, member 2 -3.128 -3.354   
133 NM_000104 B OC   CYP1B1 Cytochrome P450, family 1, subfamily B, 1 -3.075 -5.148   
136 NM_003005 B    SELP Selectin P 8.740 3.066   
139 NM_004334 B OC   BST1 Bone marrow stromal cell antigen 1 (CD157) 3.037 3.601   
141 AK023619 A    CRIM1 Cysteine-rich motor neuron 1 -3.043 -3.271   
143 NM_013332 A   WNT HIG2 Hypoxia-inducible protein 2 -6.019 -3.035   
144 NM_001845 A, B   TGF-β/BMP COL4A1 Collagen, type IV, alpha 1 -3.028 -8.960 COL4A1 0.57
146 NM_016224 A, B OB WNT SNX9 Sorting nexin 9 -3.025 -6.086   
  1. Rank' indicates the ranking within the top 150 differentially expressed genes in osteoarthritis (OA) bone compared with control (CTL) and osteoporosis (OP) bone. 'Role' indicates the known or suspected role of the gene: A, angiogenic; B, osteogenic; and C, chondrogenic. 'Cell type' indicates the cell type that the gene is expressed in or affects: OB, osteoblast, OC, osteoclast, OS, osteocyte, CB, chondroblast, or M, monocyte. 't OA/CTL' is the t score of OA compared with CTL differential expression of gene: a positive value indicates upregulation in OA and a negative one indicates downregulation in OA. 't OA/OP' is the t score of OA compared with OP differential expression of gene: a positive value indicates upregulation in OA and a negative one indicates downregulation in OA. The moderated t-statistic score is based on the ratio of the log2 fold change to its standard error. 'OA/CTL' under 'Real-time PCR' indicates the fold change in gene expression expressed as ratio of OA to CTL.