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Table 4 WNT and TGF-β/BMP signalling pathway components and target genes differentially expressed in OA bone

From: Microarray gene expression profiling of osteoarthritic bone suggests altered bone remodelling, WNT and transforming growth factor-β/bone morphogenic protein signalling

        Real-time PCR
GenBank Role Cell type Symbol Name t OA/CTL t OA/OP Symbol OA/CTL
WNT pathway components and modulators
AY009399 B, C OB, OC, CB WNT5B Wingless-type MMTV integration site family, member 5B 3.719 0.717 WNT5B 2.52
NM_002332 A, B, C OB, OC, CB LRP1 Low density lipoprotein-related protein 1 3.062 -7.071   
NM_002333    LRP3 Low density lipoprotein-related protein 3 5.950 1.146   
NM_017412    FZD3 Frizzled homolog 3 -3.677 0.835   
AF086500    FZD8 Frizzled homolog 8 3.024 -1.557   
NM_003015    SFRP5 Secreted frizzled-related protein 5 -4.338 1.154   
NM_004655    AXIN2 Axin 2 -3.123 2.490   
NM_000038    APC Adenomatosis polyposis coli -5.171 1.155   
AK023892    DAAM1 Dishevelled associated activator of morphogenesis 1 -6.704 2.850   
NM_000314 A, B OB, OC PTEN Phosphatase and tensin homolog -5.288 -0.216 PTEN 0.30
AF028823    TIP1 Tax interaction protein 1 5.057 -5.674   
AB006630    TCF20 Transcription factor 20 8.559 2.719   
AK026898 B M FOXP1 Forkhead box P1 -5.122 1.761   
WNT inducible/target genes
NM_000591 B OB, OC, M CD14 CD14 antigen 5.965 -7.036 CD14 3.47
NM_004039 A, B OB, OC, M ANXA2 Annexin A2 5.716 -7.601   
NM_000211 B OC ITGB2 Integrin, beta 2 (CD18) 6.104 -1.243 ITGB2 2.95
NM_018456    EAF2 ELL associated factor 2 6.450 0.884   
NM_000041 B OB, M APOE Apolipoprotein E 6.896 -2.391   
AB012643 B OB ALPL Alkaline phosphatase, liver/bone/kidney -5.942 -1.213   
NM_002229 B OB JUNB Jun B proto-oncogene 5.053 -3.804   
NM_003377 A, B   VEGFB Vascular endothelial growth factor B 3.238 -3.772   
NM_005429 A, B   VEGFC Vascular endothelial growth factor C 5.304 0.473   
NM_000963 B OB PTGS2 Prostaglandin-endoperoxide synthase 2 4.346 -0.842   
NM_005194 B OB CEBPB CCAAT/enhancer binding protein, beta 4.273 -4.092   
AL353944 A, B OB ID1 Inhibitor of DNA binding 1 3.923 -1.996   
NM_000900 B OB MGP Matrix Gla protein 2.791 -3.073   
M14584 A, B, C OB, OC, CB IL6 Interleukin 6 2.589 -3.612   
NM_006350 B, C OB, CB FST Follistatin -2.137 -7.912 FST 0.38
AL353944 B OB RUNX2 Runt-related transcription factor 2 1.611 1.050 RUNX2 2.07
TGF-β/BMP pathway components and modulators
M38449 A, B, C OB, OC, CB TGFB1 Transforming growth factor, beta 1 2.739 3.054   
AK021486 B, C OB, CB BMP5 Bone morphogenetic protein 5 -10.314 -2.263   
NM_002192 A, B, C OB, OC, CB INHBA Inhibin, beta A -7.699 -0.389 INHBA 0.31
NM_006350 B, C OB, CB FST Follistatin -2.137 -7.912 FST 0.38
NM_004612 B   TGFBR1 Transforming growth factor, beta receptor I -4.915 1.049   
NM_000118 A   ENG Endoglin 4.131 -2.461   
NM_001105 B OB ACVR1 Activin A receptor, type I 3.791 -0.941   
NM_003573    LTBP4 Latent transforming growth factor beta binding protein 4 -4.060 2.623   
NM_005902 A, B OB SMAD3 SMAD, mothers against DPP homolog 3 4.119 -3.582 SMAD3 2.64
NM_005359 A, B OB SMAD4 SMAD, mothers against DPP homolog 4 3.957 0.156   
NM_002165 A, B OB ID1 Inhibitor of DNA binding 1 3.923 -1.996   
NM_002229 B OB JUNB Jun B proto-oncogene 5.053 -3.804   
NM_005655 B OB KLF10 Kruppel-like factor 10 4.443 -4.449   
NM_006037 B OB HDAC4 Histone deacetylase 4 8.475 2.468   
NM_000168 B OB GLI3 GLI-Kruppel family member GLI3 -4.675 -0.298   
AL353944 B OB RUNX2 Runt-related transcription factor 2 1.611 1.050 RUNX2 2.07
TGF-β/BMP inducible/target genes
AK001052 B OB FGFR1 Fibroblast growth factor receptor 1 4.234 1.993   
NM_001553 A   IGFBP7 Insulin-like growth factor binding protein 7 5.137 -6.302   
NM_000211 B OC ITGB2 Integrin, beta 2 (CD18) 6.104 -1.243 ITGB2 2.95
AK001060 B, C OB, CB DCN Decorin -6.325 2.628   
NM_000177 B OC GSN Gelsolin 4.725 -2.387   
X55525 B OB COL1A2 Collagen, type I, alpha 2 5.538 -3.633   
NM_003118 A, B OB SPARC Secreted protein, acidic, cysteine-rich 3.188 -1.844   
NM_004407 B OB, OS DMP1 Dentin matrix acidic phosphoprotein -3.523 -1.397   
NM_001831 A, B   CLU Clusterin 4.803 -2.030   
Not represented on Compugen human 19K microarray
NM_001904 A, B OB, OC CTNNB1 Catenin beta 1    CTNNB1 2.38
  1. 'Role' indicates the known or suspected role of the gene: A, angiogenic; B, osteogenic; and C, chondrogenic. 'Cell type' indicates the cell type that the gene is expressed in or affects: OB, osteoblast, OC, osteoclast, OS, osteocyte, CB, chondroblast, or M, monocyte. 't OA/CTL' is the t score of osteoarthritis (OA) compared with control (CTL) differential expression of gene: a positive value indicates upregulation in OA and a negative one indicates downregulation in OA. 't OA/OP' is the t score of OA compared with osteoporosis (OP) differential expression of gene: a positive value indicates upregulation in OA and a negative one indicates downregulation in OA. The moderated t-statistic score is based on the ratio of the log2 fold change to its standard error. 'OA/CTL' under 'Real-time PCR' indicates the fold change in gene expression expressed as ratio of OA to CTL. BMP, bone morphogenic protein; TGF, transforming growth factor.