Table 4 WNT and TGF-β/BMP signalling pathway components and target genes differentially expressed in OA bone
Real-time PCR | ||||||||
|---|---|---|---|---|---|---|---|---|
GenBank | Role | Cell type | Symbol | Name | t OA/CTL | t OA/OP | Symbol | OA/CTL |
WNT pathway components and modulators | ||||||||
AY009399 | B, C | OB, OC, CB | WNT5B | Wingless-type MMTV integration site family, member 5B | 3.719 | 0.717 | WNT5B | 2.52 |
NM_002332 | A, B, C | OB, OC, CB | LRP1 | Low density lipoprotein-related protein 1 | 3.062 | -7.071 | ||
NM_002333 | LRP3 | Low density lipoprotein-related protein 3 | 5.950 | 1.146 | ||||
NM_017412 | FZD3 | Frizzled homolog 3 | -3.677 | 0.835 | ||||
AF086500 | FZD8 | Frizzled homolog 8 | 3.024 | -1.557 | ||||
NM_003015 | SFRP5 | Secreted frizzled-related protein 5 | -4.338 | 1.154 | ||||
NM_004655 | AXIN2 | Axin 2 | -3.123 | 2.490 | ||||
NM_000038 | APC | Adenomatosis polyposis coli | -5.171 | 1.155 | ||||
AK023892 | DAAM1 | Dishevelled associated activator of morphogenesis 1 | -6.704 | 2.850 | ||||
NM_000314 | A, B | OB, OC | PTEN | Phosphatase and tensin homolog | -5.288 | -0.216 | PTEN | 0.30 |
AF028823 | TIP1 | Tax interaction protein 1 | 5.057 | -5.674 | ||||
AB006630 | TCF20 | Transcription factor 20 | 8.559 | 2.719 | ||||
AK026898 | B | M | FOXP1 | Forkhead box P1 | -5.122 | 1.761 | ||
WNT inducible/target genes | ||||||||
NM_000591 | B | OB, OC, M | CD14 | CD14 antigen | 5.965 | -7.036 | CD14 | 3.47 |
NM_004039 | A, B | OB, OC, M | ANXA2 | Annexin A2 | 5.716 | -7.601 | ||
NM_000211 | B | OC | ITGB2 | Integrin, beta 2 (CD18) | 6.104 | -1.243 | ITGB2 | 2.95 |
NM_018456 | EAF2 | ELL associated factor 2 | 6.450 | 0.884 | ||||
NM_000041 | B | OB, M | APOE | Apolipoprotein E | 6.896 | -2.391 | ||
AB012643 | B | OB | ALPL | Alkaline phosphatase, liver/bone/kidney | -5.942 | -1.213 | ||
NM_002229 | B | OB | JUNB | Jun B proto-oncogene | 5.053 | -3.804 | ||
NM_003377 | A, B | VEGFB | Vascular endothelial growth factor B | 3.238 | -3.772 | |||
NM_005429 | A, B | VEGFC | Vascular endothelial growth factor C | 5.304 | 0.473 | |||
NM_000963 | B | OB | PTGS2 | Prostaglandin-endoperoxide synthase 2 | 4.346 | -0.842 | ||
NM_005194 | B | OB | CEBPB | CCAAT/enhancer binding protein, beta | 4.273 | -4.092 | ||
AL353944 | A, B | OB | ID1 | Inhibitor of DNA binding 1 | 3.923 | -1.996 | ||
NM_000900 | B | OB | MGP | Matrix Gla protein | 2.791 | -3.073 | ||
M14584 | A, B, C | OB, OC, CB | IL6 | Interleukin 6 | 2.589 | -3.612 | ||
NM_006350 | B, C | OB, CB | FST | Follistatin | -2.137 | -7.912 | FST | 0.38 |
AL353944 | B | OB | RUNX2 | Runt-related transcription factor 2 | 1.611 | 1.050 | RUNX2 | 2.07 |
TGF-β/BMP pathway components and modulators | ||||||||
M38449 | A, B, C | OB, OC, CB | TGFB1 | Transforming growth factor, beta 1 | 2.739 | 3.054 | ||
AK021486 | B, C | OB, CB | BMP5 | Bone morphogenetic protein 5 | -10.314 | -2.263 | ||
NM_002192 | A, B, C | OB, OC, CB | INHBA | Inhibin, beta A | -7.699 | -0.389 | INHBA | 0.31 |
NM_006350 | B, C | OB, CB | FST | Follistatin | -2.137 | -7.912 | FST | 0.38 |
NM_004612 | B | TGFBR1 | Transforming growth factor, beta receptor I | -4.915 | 1.049 | |||
NM_000118 | A | ENG | Endoglin | 4.131 | -2.461 | |||
NM_001105 | B | OB | ACVR1 | Activin A receptor, type I | 3.791 | -0.941 | ||
NM_003573 | LTBP4 | Latent transforming growth factor beta binding protein 4 | -4.060 | 2.623 | ||||
NM_005902 | A, B | OB | SMAD3 | SMAD, mothers against DPP homolog 3 | 4.119 | -3.582 | SMAD3 | 2.64 |
NM_005359 | A, B | OB | SMAD4 | SMAD, mothers against DPP homolog 4 | 3.957 | 0.156 | ||
NM_002165 | A, B | OB | ID1 | Inhibitor of DNA binding 1 | 3.923 | -1.996 | ||
NM_002229 | B | OB | JUNB | Jun B proto-oncogene | 5.053 | -3.804 | ||
NM_005655 | B | OB | KLF10 | Kruppel-like factor 10 | 4.443 | -4.449 | ||
NM_006037 | B | OB | HDAC4 | Histone deacetylase 4 | 8.475 | 2.468 | ||
NM_000168 | B | OB | GLI3 | GLI-Kruppel family member GLI3 | -4.675 | -0.298 | ||
AL353944 | B | OB | RUNX2 | Runt-related transcription factor 2 | 1.611 | 1.050 | RUNX2 | 2.07 |
TGF-β/BMP inducible/target genes | ||||||||
AK001052 | B | OB | FGFR1 | Fibroblast growth factor receptor 1 | 4.234 | 1.993 | ||
NM_001553 | A | IGFBP7 | Insulin-like growth factor binding protein 7 | 5.137 | -6.302 | |||
NM_000211 | B | OC | ITGB2 | Integrin, beta 2 (CD18) | 6.104 | -1.243 | ITGB2 | 2.95 |
AK001060 | B, C | OB, CB | DCN | Decorin | -6.325 | 2.628 | ||
NM_000177 | B | OC | GSN | Gelsolin | 4.725 | -2.387 | ||
X55525 | B | OB | COL1A2 | Collagen, type I, alpha 2 | 5.538 | -3.633 | ||
NM_003118 | A, B | OB | SPARC | Secreted protein, acidic, cysteine-rich | 3.188 | -1.844 | ||
NM_004407 | B | OB, OS | DMP1 | Dentin matrix acidic phosphoprotein | -3.523 | -1.397 | ||
NM_001831 | A, B | CLU | Clusterin | 4.803 | -2.030 | |||
Not represented on Compugen human 19K microarray | ||||||||
NM_001904 | A, B | OB, OC | CTNNB1 | Catenin beta 1 | CTNNB1 | 2.38 | ||