Retroviral expression of SOX9 in SW1353 chondrosarcoma cells. (a) Phase contrast micrograph demonstrating the morphology of SW1353 chondrosarcoma cells transduced with a retrovirus containing GFP or bicistronic SOX9-GFP. Scale Bar = 50 μm. (b) Cell lysates from GFP- or SOX9-SW1353 cells were analysed by western blotting using an anti-SOX9 antibody. (c) Real time PCR analysis of cDNA derived from green-fluorescent protein (GFP; black bars) or bicistronic SOX9-GFP (grey bars) transduced SW1353 chondrosarcoma cells.