Figure 2From: Differential expression of the FAK family kinases in rheumatoid arthritis and osteoarthritis synovial tissuesRheumatoid arthritis synovial tissue (RA ST) had higher pPyk2 immunopositive cells compared to osteoarthritis (OA) ST. (a) RA (×200), compared to (b) OA (×200) and (c) normal donor (ND) (×200). (d) is the quantification data obtained from figure a and b. Bars represent mean and SEM. RA ST fibroblasts (e and f) or peripheral blood differentiated MΦs (g and h) were stimulated with TNF-α (10 ng/ml) or IL1-β (10 ng/ml) from 0–120 min. Cell lysates were examined by western blot analysis for pPyk2 or Pyk2 expression. The results are representative of three experiments. Inflam, inflammatory score; Vasc, vascularity score; Lining, ST lining cell layer; Mac, subsynovial MΦs.Back to article page