Figure 3
From: Analysis of C4 and the C4 binding protein in the MRL/lpr mouse
Similar serum autoantibody titers and circulating immune complexes in knockout MRL and control MRL mice. (a) Sera from C4bp-/-MRL/lpr (KO MRL) mice (â–², solid line, n = 15) and littermate control (CTRL MRL) mice (â—†, dashed line, n = 8) were tested for binding to double-stranded DNA by ELISA using serial serum dilution (x axis). Pooled normal mouse serum from nonautoimmune mice (â– , NMS) was used as a negative control. P > 0.05, KO MRL mice versus CTRL MRL mice. OD450, optical density at 450 nm. (b) Sera from 20-week-old KO MRL mice (â–², n = 15) and littermate control mice (â—†, CTRL MRL, n = 8) were tested for binding to human C1q by ELISA. Data for each individual mouse are shown and mean increases in immune complex levels are displayed as solid lines. P > 0.05, KO MRL mice versus CTRL MRL mice. AU, arbitrary units.