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Figure 5 | Arthritis Research & Therapy

Figure 5

From: Activation of proteinase-activated receptor 2 in human osteoarthritic cartilage upregulates catabolic and proinflammatory pathways capable of inducing cartilage degradation: a basic science study

Figure 5

Signalling pathways induced by specific proteinase-activated receptor 2 (PAR-2) activation. Representative Western blot of time and dose curves of PAR-2 signalling in osteoarthritic chondrocytes (n = 3 or 4). Phosphorylated forms of Erk1/2 (p-Erk1/2) (a,b), p38 (p-p38) (c,d), and JNK (p-JNK) (e,f) treated with PAR-2-activating peptide (PAR-2-AP) at 0 (-), 10, 100, and 400 μM in the absence (a,c,e) or presence (b,d,f) of interleukin 1 beta (IL-1β) (100 pg/mL) for 0 to 60 minutes (a,c,e). P values indicate the comparison between the untreated (-) and the PAR-2-AP-treated chondrocytes. For p-Erk1/2, all of the times and concentrations showed a statistically significant increase (p < 0.03). For p-p38, statistical significance was reached for each concentration at 5 and 15 minutes (p < 0.03). (b,d,f) P values indicate the comparison between the untreated and the IL-1β-treated chondrocytes in the absence or presence of PAR-2-AP. Erk1/2, extracellular signal-regulated kinase 1/2; JNK, c-jun N-terminal kinase.

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Arthritis Research & Therapy

ISSN: 1478-6362

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