Table 1 Gene-specific primer pairs used in real-time quantitative polymerase chain reaction

Aggrecan

5'-ACA CCC CTA CCC TTG CTT CT-3'

5'-AAA GTG TCC AAG GCA TCC AC-3'

Ank

5'-CAA GAG AGA CAG GGC CAA AG-3'

5'-AAG GCA GCG AGA TAC AGG AA-3'

L27

5'-TCC TGG CTG GAC GCT ACT C-3'

5'-CCA CAG AGT ACC TTG TGG GC-3'

PC-1

5'-TAT GCC CAA GAA AGG AAT GG-3'

5'-GCA GCT GGT AAG CAC AAT GA-3'

S29

5'-AAG ATG GGT CAC CAG CAG CTC TAC TG-3'

5'-AGA CGC GGC AAG AGC GAG AA-3'

TNAP

5'-GAA CGT CAA TTA ACG GCT GA-3'

5'-CAG ATG GGT GGG AAG AGG T-3'

Type IA2 collagen

5'-TTG ACC CTA ACC AAG GAT GC-3'

5'-CAC CCC TTC TGC GTT GTA TT-3'

Type II collagen

5'-TCC CTC TGG TTC TGA TGG TC-3'

5'-CTC TGT CTC CAG ATG CAC CA-3'

Type X collagen

5'-ATA TCC TGG GGA TCC AGG TC-3'

5'-TGG GTC ACC CTT AGA TCC AG-3'