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Table 1 Gene-specific primer pairs used in real-time quantitative polymerase chain reaction

From: Inorganic pyrophosphate generation by transforming growth factor-beta-1 is mainly dependent on ANK induction by Ras/Raf-1/extracellular signal-regulated kinase pathways in chondrocytes

Gene Sense Antisense Amplicon length (base pair) GenBank accession number
Aggrecan 5'-ACA CCC CTA CCC TTG CTT CT-3' 5'-AAA GTG TCC AAG GCA TCC AC-3' 124 NM_022190
Ank 5'-CAA GAG AGA CAG GGC CAA AG-3' 5'-AAG GCA GCG AGA TAC AGG AA-3' 173 NM_053714
L27 5'-TCC TGG CTG GAC GCT ACT C-3' 5'-CCA CAG AGT ACC TTG TGG GC-3' 227 NM_022514
PC-1 5'-TAT GCC CAA GAA AGG AAT GG-3' 5'-GCA GCT GGT AAG CAC AAT GA-3' 165 NM_053535
S29 5'-AAG ATG GGT CAC CAG CAG CTC TAC TG-3' 5'-AGA CGC GGC AAG AGC GAG AA-3' 67 NM_012876
TNAP 5'-GAA CGT CAA TTA ACG GCT GA-3' 5'-CAG ATG GGT GGG AAG AGG T-3' 50 NM_013059
Type IA2 collagen 5'-TTG ACC CTA ACC AAG GAT GC-3' 5'-CAC CCC TTC TGC GTT GTA TT-3' 197 NM_053356
Type II collagen 5'-TCC CTC TGG TTC TGA TGG TC-3' 5'-CTC TGT CTC CAG ATG CAC CA-3' 161 NM_012929
Type X collagen 5'-ATA TCC TGG GGA TCC AGG TC-3' 5'-TGG GTC ACC CTT AGA TCC AG-3' 241 AJ131848
  1. TNAP, tissue-nonspecific alkaline phosphatase.