Figure 7
PPI-2458 does not affect HUVEC inflammatory responses. Human umbilical vein endothelial cells (HUVEC) were plated at 3 × 104 in 75 mm2 wells and stimulated for 24 hours with either (a) IL-1β (10 ng/ml), (b) tumour necrosis factor (TNF)-α (10 ng/ml), or (c) lipopolysaccharide (LPS; 500 ng/ml). Fumagillin (10 nmol/l) and PPI-2458 (0.01 to 10 nmol/l), or 0.001% dimethyl sulfoxide as a vehicle control were added where indicated. Release of monocyte chemoattractant protein (MCP)-1 was measured by enzyme-linked immunosorbent assay (ELISA). MCP-1 was measured by ELISA. Data are expressed as mean ± standard deviation, representative of at least three experiments.