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Table 2 Effect of CD47/IAP transfection on the electrophysiological response of CD47-null cells to mechanical stimulation

From: CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitromodel

   Membrane potential (-mV) (mean ± SEM)  
Frequency of stimulation Cell (phenotype) Resting Post mechanical stimulation Percentage change
0.33 Hz 3657L (CD47 wild-type+) 10.9 ± 0.27 4.5 ± 0.64 -59a
  3656 1/Sra (CD47-null) 14.0 ± 1.36 13.8 ± 0.46 -1b
  3656 1/171 (mouse CD47 type 2+) 13.1 ± 0.52 5.9 ± 0.65 -55a
  3656 1/315 (human CD47 type 2+) 11.8 ± 1.03 4.6 ± 1.03 -61a
0.083 Hz 3657L (CD47 wild-type+) 10.8 ± 0.6 11.9 ± 0.72 +10b
  3656 1/Sra (CD47-null) 12.4 ± 0.43 13.0 ± 0.27 +5b
  3656 1/171 (mouse CD47 type 2+) 12.8 ± 0.53 20.4 ± 0.92 +48a
  3656 1/315 (human CD47 type 2+) 12.2 ± 0.91 17.2 ± 0.32 +42a
  OV10 58A2 (CD47-null) 10.2 ± 0.85 10.8 ± 1.50 +6b
  OV10 315 (human CD47 type 2+) 7.0 ± 0.84 18.6 ± 1.86 +165a
  OV10 164/315 (β3/CD47 type 2+) 35.4 ± 1.6 49.2 ± 3.34 +39a
  OV10 148A10T (CD47IgV/GPI) 22.6 ± 1.33 22.2 ± 1.07 0b
  1. CD47-null cells or cells transfected with either type 2 CD47 cDNA, CD47 extracellular immunoglobulin variable (IgV) domain linked to a glycosylphosphatidylinositol anchor, or vector control were studied. Membrane potentials of cells were measured at rest and after 20 minutes of mechanical stimulation at 0.33 or 0.083 Hz. N = 5. ap ≤ 0.01; bnot significant; SEM, standard error of the mean.