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Table 1 Primers used for semi-quantitative PCR and product length

From: Anti-inflammatory effect of antidiabetic thiazolidinediones prevents bone resorption rather than cartilage changes in experimental polyarthritis

Gene encoding

Primer sequence

Size (base pairs)

Cycles

Tm (°C)

L27

Sense: 5'-TCCTGGCTGGACGCTACTC-3'

225

27

62

 

Antisense: 5'-CCACAGAGTACCTTGTGGGC-3'

   

MCP-1

Sense: 5'-ATGCAGTTAATGCCCCACTC-3'

167

29

57

 

Antisense: 5'-TTCCTTATTGGGGTCAGCAC-3'

   

bFGF

Sense: 5'-GAACCGGTACCTGGCTATGA-3'

182

31

61

 

Antisense: 5'-CCGTTTTGGATCCGAGTTTA-3'

   

VEGF

Sense: 5'-CAATGATGAAGCCCTGGAGT-3'

211

32

64

 

Antisense: 5'-TTTCTTGCGCTTTCGTTTTT-3'

   

TNF-α

Sense: 5'-AGATGTGGAACTGGCAGAGG-3'

178

31

58

 

Antisense: 5'-CCCATTTGGGAACTTCTCCT-3'

   

IL-1β

Sense: 5'-TGAAAGCTCTCCACCTCAATGG-3'

366

28

61

 

Antisense: 5'-TCCATGGTGAAGTCAACTATGTCC-3'

   
  1. MCP-1, monocyte chemotactic protein-1; bFGF, basic fibroblast growth factor; VEGF, vascular endothelial growth factor; Tm, melting temperature.
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Arthritis Research & Therapy

ISSN: 1478-6362

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