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Table 1 Primers used for semi-quantitative PCR and product length

From: Anti-inflammatory effect of antidiabetic thiazolidinediones prevents bone resorption rather than cartilage changes in experimental polyarthritis

Gene encoding Primer sequence Size (base pairs) Cycles Tm (°C)
L27 Sense: 5'-TCCTGGCTGGACGCTACTC-3' 225 27 62
  Antisense: 5'-CCACAGAGTACCTTGTGGGC-3'    
MCP-1 Sense: 5'-ATGCAGTTAATGCCCCACTC-3' 167 29 57
  Antisense: 5'-TTCCTTATTGGGGTCAGCAC-3'    
bFGF Sense: 5'-GAACCGGTACCTGGCTATGA-3' 182 31 61
  Antisense: 5'-CCGTTTTGGATCCGAGTTTA-3'    
VEGF Sense: 5'-CAATGATGAAGCCCTGGAGT-3' 211 32 64
  Antisense: 5'-TTTCTTGCGCTTTCGTTTTT-3'    
TNF-α Sense: 5'-AGATGTGGAACTGGCAGAGG-3' 178 31 58
  Antisense: 5'-CCCATTTGGGAACTTCTCCT-3'    
IL-1β Sense: 5'-TGAAAGCTCTCCACCTCAATGG-3' 366 28 61
  Antisense: 5'-TCCATGGTGAAGTCAACTATGTCC-3'    
  1. MCP-1, monocyte chemotactic protein-1; bFGF, basic fibroblast growth factor; VEGF, vascular endothelial growth factor; Tm, melting temperature.
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Arthritis Research & Therapy

ISSN: 1478-6362

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