Effects of HSP60 and LPS on HO-1 protein expression in PBMCs from patients with BD. (a) Effect of lipopolysaccharide (LPS) stimulation on heme oxygenase (HO)-1 and actin protein expression in peripheral blood mononuclear cells (PBMCs) from patients with Behçet's disease (BD). PBMCs from a BD patient were stimulated with LPS in the presence or absence of 10 ng/ml interleukin (IL)-10 and 100 μg/ml polymyxin B (PMB). Representative immunoblotting data for HO-1 protein in the cells are shown. The arrowhead indicates 32 kDa molecular weight HO-1 specific band. (b) Effect of heat shock protein (HSP)60 (3 μg/ml) stimulation on endogenous HO-1 protein expression in PBMCs from patients with BD. The arrowhead indicates 32 kDa HO-1 specific band. A representative of three independent experiments is shown. (c) Mean and standard error of the mean (SEM) values of HO-1 and actin protein expression in PBMCs stimulated by LPS (1 ng/ml) for 24 hours in patients with BD (n = 14). #P < 0.001, as determined using paired t-test. (d) Effect of infliximab (10 μg/ml) or IgG1κ (10 μg/ml) on HO-1 expression in LPS (10 ng/ml) or tumor necrosis factor (TNF; 1 ng/ml) treated PBMCs.