Effect of forced HO-1 expression on Toll-like receptor (TLR)2 and TLR4 mRNA expression in peripheral monocytes. (a) Immunoblotting analysis of heme oxygenase (HO)-1 and actin in pHO-1 (human HO-1 expression vector) or pCont (control vector) transfected monocytes. The arrow represents HO-1 protein. (b) Real-time PCR analysis of TLR2 and TLR4 mRNA expression in pHO-1 transfected peripheral blood mononuclear cells (PBMCs). NS, not significant.