Resting CD4+effector memory T cells are precursors of bystander-activated effectors: a surrogate model of rheumatoid arthritis synovial T-cell function

Table 1 Expression of activation markers, adhesion molecules and chemokine receptors in CD4⁺CD45RO⁺ Tck cells

Cell phenotype	Median MFI (range)/percentage positive (%) index		Change^a	P	n
	Day 0	Day 8
CD25	8.0 (2–49)/9%	61 (6–884)/18%	7.5	<0.0001	40
CD69	5.0 (2–43)/1.1%	41 (5–552)/26.7%	8	<0.0001	41
HLA-DR	19 (4–130)/9%	60 (11–238)/15%	3	0.0052	15
CD62L	22 (8–255)/17%	339 (7–1,040)/58%	15	<0.0001	31
CD18	107 (9–1,026)/97.5%	319 (53–2,040)/98.5%	3	0.0085	21
CD29	85 (6–668)/81%	152 (14–582)/97%	2	0.0132	35
CD49d	180 (5–634)/73.3%	465 (13–1,461)/82.3%	2.6	<0.0001	42
CCR7	12 (4–57)/7.4%	13 (3–138)/8.6%	1	NS	42
CXCR4	52 (18–494)/29%	270 (124–896)/86.4%	5	<0.0001	21
CXCR3	32.3 (9–309)/34.3%	40.4 (17–129)/34.0%	1	NS	21
CCR5	8.3 (4–28)/2%	10.7 (4–262)/6%	1	NS	21

Statistically significant differences in mean fluorescence intensity (MFI) between day 0 and day 8 values from different experiments were calculated using Wilcoxon rank test. ^aChange index is calculated with reference to Day 0. CCR, CC chemokine receptor; CXCR, CXC chemokine receptor; HLA, human leucocyte antigen; NS, not significant; Tck, cytokine-activated T.

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