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Figure 4 | Arthritis Research & Therapy

Figure 4

From: Prolonged, granulocyte–macrophage colony-stimulating factor-dependent, neutrophil survival following rheumatoid synovial fibroblast activation by IL-17 and TNFalpha

Figure 4

Soluble, temperature-sensitive factors released by stimulated rheumatoid arthritis synovial fibroblasts extend neutrophil survival. (a) Peripheral blood neutrophils were either cocultured with fibroblasts (Fb), with conditioned medium from IL-17 and TNFα pretreated fibroblasts (FCM), or on a transwell filter suspended above fibroblasts (Tw) for 24 hours. Error bars show the mean ± standard deviation from three independent experiments. **P < 0.01, *P < 0.05; ns, nonsignificant. (b) Culture supernatant from rheumatoid arthritis synovial fibroblasts stimulated with IL-17 and TNFα was heated to 92°C for the times indicted before culture with neutrophils for 24 hours, and neutrophil survival was measured. Error bars show the mean ± standard deviation from three independent experiments

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