Action of fibroblast growth factor-2 on the intervertebral disc

Table 1 Primer sequences for RT-PCR

Gene	Primer sequence (forward/reverse) (5' to 3')	Size (base pairs)	Annealing temperature (°C)	Reference accession number
h-FGFR1	AAC CCC AGC CAC AAC, CCA AAG CTG GGC TGG GTG TCG	687	60	EMBL: NM_015850.2
h-FGF2	GAG AAG AGC GAC CCT CAC A TAG CTT TCT GCC CAG GTC C	278	58	EMBL: NM_002006.3
h-ADAMTS5	CCC TCT TCC CTG TGC AGT AG CTA CGA TGC CAC CCA GCA G	363	58	EMBL: NM_007038.2
h-MMP-13	GGC TCC GAG AAA TGC AGT CTT TCT T ATC AAA TGG GTA GAA GTC GCC ATG C	338	0	EMBL: NM_007038.2
h-GAPDH	GGT ATC GTG GAA GGA CTC AT ACC ACC TGG TGC TCA GTG TA	340	55	EMBL: XR_018317.1
Bov-MMP-13	ACC CTT CCT TAT CCC TTG ATG CCA AAA CAG CTC TGC TTC AAC CTG CTG	110	55	EMBL: NM_174389.2
Bov-ADAMTS 4	ACT GGG CTA CTA TTA CGT GGA AAA CAC ACA CCA TGC ACT TGTCGA ACT	155	60	EMBL: BC148059.1
Bov-ADAMTS 5	ACG TGG TGT TCT CTC CAA AG CAT ACT GCA GCT TCG AGC CA	146	55	EMBL: XM_589193.2
Bov-FGFR1	AGG TAA CAA GAA GAC AAG CGG GCA ATG GGC CAG TAA GTG AAG ACC ACT	127	55	EMBL: XM_001255761.1
Bov-FGFR2	ATA CCT GCG TGG TGG AGA ACG ATT TTT GCA GAC AAA CTC CAC ATC GCC	152	55	EMBL: XM_880481.2
Bov-FGFR3	GTG GCC GTG AAG ATG CTG AAA GAT AGG CGC CTA GCA GGT TGA TAA TGT	120	55	EMBL: NM_174318.2
Bov-FGFR4	GCT GAT TGG CCG ACA CAA GAA CAT AGC ACA CTC CAC GAT CAC GTA CAA	85	55	EMBL: XM_602166.3
Bov-noggin	TCT GTA ACT TCC TCC GCA GCT TCT AGC GAG ATC AAA GCG CTG GAG TT	88	55	EMBL: XM_582573.3
Bov-β-actin	AAG AGA TCA ATG ACC TGG CAC CCA ACT CCT GCT TGC TGA TCC ACA TCT	141	55	EMBL: BT030480.1

ADAMTS = a disintegrin and metalloproteinase with thrombospondin motifs; FGF2 = fibroblast growth factor 2; FGFR = fibroblast growth factor receptor; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; MMP = matrix metalloprotease.

ISSN: 1478-6362