Skip to main content

Table 1 Primer sequences for RT-PCR

From: Action of fibroblast growth factor-2 on the intervertebral disc

Gene Primer sequence (forward/reverse) (5' to 3') Size (base pairs) Annealing temperature (°C) Reference accession number
h-FGFR1 AAC CCC AGC CAC AAC, CCA
AAG CTG GGC TGG GTG TCG
687 60 EMBL: NM_015850.2
h-FGF2 GAG AAG AGC GAC CCT CAC A
TAG CTT TCT GCC CAG GTC C
278 58 EMBL: NM_002006.3
h-ADAMTS5 CCC TCT TCC CTG TGC AGT AG
CTA CGA TGC CAC CCA GCA G
363 58 EMBL: NM_007038.2
h-MMP-13 GGC TCC GAG AAA TGC AGT CTT TCT T
ATC AAA TGG GTA GAA GTC GCC ATG C
338 0 EMBL: NM_007038.2
h-GAPDH GGT ATC GTG GAA GGA CTC AT
ACC ACC TGG TGC TCA GTG TA
340 55 EMBL: XR_018317.1
Bov-MMP-13 ACC CTT CCT TAT CCC TTG ATG CCA
AAA CAG CTC TGC TTC AAC CTG CTG
110 55 EMBL: NM_174389.2
Bov-ADAMTS 4 ACT GGG CTA CTA TTA CGT GGA AAA
CAC ACA CCA TGC ACT TGTCGA ACT
155 60 EMBL: BC148059.1
Bov-ADAMTS 5 ACG TGG TGT TCT CTC CAA AG
CAT ACT GCA GCT TCG AGC CA
146 55 EMBL: XM_589193.2
Bov-FGFR1 AGG TAA CAA GAA GAC AAG CGG GCA
ATG GGC CAG TAA GTG AAG ACC ACT
127 55 EMBL: XM_001255761.1
Bov-FGFR2 ATA CCT GCG TGG TGG AGA ACG ATT
TTT GCA GAC AAA CTC CAC ATC GCC
152 55 EMBL: XM_880481.2
Bov-FGFR3 GTG GCC GTG AAG ATG CTG AAA GAT
AGG CGC CTA GCA GGT TGA TAA TGT
120 55 EMBL: NM_174318.2
Bov-FGFR4 GCT GAT TGG CCG ACA CAA GAA CAT
AGC ACA CTC CAC GAT CAC GTA CAA
85 55 EMBL: XM_602166.3
Bov-noggin TCT GTA ACT TCC TCC GCA GCT TCT
AGC GAG ATC AAA GCG CTG GAG TT
88 55 EMBL: XM_582573.3
Bov-β-actin AAG AGA TCA ATG ACC TGG CAC CCA
ACT CCT GCT TGC TGA TCC ACA TCT
141 55 EMBL: BT030480.1
  1. ADAMTS = a disintegrin and metalloproteinase with thrombospondin motifs; FGF2 = fibroblast growth factor 2; FGFR = fibroblast growth factor receptor; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; MMP = matrix metalloprotease.