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Table 5 Validation of array data by real-time quantitative RT-PCR

From: Molecular discrimination of responders and nonresponders to anti-TNFalpha therapy in rheumatoid arthritis by etanercept

Gene

Probe set

Correlation coefficient

 

U133A

U95A

U133A versus RT-PCR (n = 19)

U133A versus U95A (n = 11)

U95A versus RT-PCR (n = 11)

ICAM1

202637_s_at

32640_at

0.9329

0.8916

0.8560

TNFAIP3

202643_s_at

595_at

0.9437

0.9537

0.9792

IL1B

39402_at

39402_at

0.9443

0.9623

0.9667

PDE4B

211302_s_at

33705_at

0.8880

0.9583

0.6307

PPP1R15A

37028_at

37028_at

0.9519

0.9869

0.7649

  1. Pearson correlation coefficients between real-time quantitative RT-PCR data (-ΔΔCT t1 versus t0) and the microarray data from the GeneChip U133A and U95A for five selected genes found to be differentially regulated in responders and nonresponders are presented.