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Figure 4 | Arthritis Research & Therapy

Figure 4

From: DREAM is reduced in synovial fibroblasts of patients with chronic arthritic pain: is it a suitable target for peripheral pain management?

Figure 4

Immunoprecipitation of DREAM protein from different tissues (arrows). The DREAM antibody recognizes the glutathione S-transferase-tagged recombinant protein (10 ng; predicted size of 54 kDa) and the native protein from neuronal tissue (human occipital cortex). The immunoprecipitations show the two antibody bands detected by the secondary goat anti-mouse antibody (heavy and light chains), and in the last lane, resembling the immunoprecipitation from neuronal tissue, a DREAM-specific signal of the expected size (~30 kDa) was detectable. DREAM, downstream regulatory element antagonist modulator; IP, immunoprecipitation; PBMC, peripheral blood mononuclear cell; SFLC, synovial fibroblast-like cell.

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Arthritis Research & Therapy

ISSN: 1478-6362

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