Table 1 Sequences of oligonucleotides used in polymerase chain reaction (PCR) and real-time PCR as well as for the generation of small interfering RNAs
Primers for conventional DREAM PCR | ||
|---|---|---|
Forward | Reverse | |
DREAM | 5'-CCGGCTAAGGAAGTGACAAA-3' | 5'-CAAAGGCGTTGAAGAGGAAG-3' |
nDREAM | 5'-GAAGGAGGGTATCAAGTG-3' | 5'-TAAATGAGTTTGAAGGTGTC-3' |
Primers for SYBR green assay real-time PCR | ||
Forward | Reverse | |
c-fos | 5'-TAAATGAGTTTGAAGGTGTC-3' | 5'-ACAGGAACCCTCTAGGGAAGA-3' |
Oligonucleotides for the synthesis of siRNAs | ||
Sense | Antisense | |
siRNA1 | 5'-AAGGACAGGATCCACTTGACCTATAGTGAGTCGTATTA-3' | 5'AAGGTCAAGTGGATCCTGTCCTATAGTGAGTCGTATTA3' |
siRNA2 | 5'-AAGGTGAACTTGGTCTGGGCCTATAGTGAGTCGTATTA3' | 5'-AAGGCCCAGACCAAGTTCACCTATAGTGAGTCGTATTA-3' |
siRNA3 | 5'-AAGTAGAGATTAAAGGCCCACTATAGTGAGTCGTATTA-3' | 5'-AAGTGGGCCTTTAATCTCTACTATAGTGAGTCGTATTA-3' |
siRNA4 | 5'-AAGCTCATGATGTTCTCATCCTATAGTGAGTCGTATTA-3' | 5'-AAGGATGAGAACATCATGAGCTATAGTGAGTCGTATTA-3' |
siRNA5 | 5'-AAGTGTAGCAATCTGTTCACTATAGTGAGTCGTATTA-3' | 5'-AAGTGAACAGATTGCTACACTATAGTGAGTCGTATTA-3' |
siRNA-GFP | 5'-ATGAACTTCAGGGTCAGCTTGCTATAGTGAGTCGTATTA-3' | 5'-CGGCAAGCTGACCCTGAAGTTCTATAGTGAGTCGTATTA-3' |
T7 | 5'-TAATACGACTCACTATAG-3' | |