Figure 3

Quantification of aggrecan and collagen degradation products at days 7, 11, and 17. Articular cartilage explants were cultured in the presence or absence of oncostatin M plus tumour necrosis factor (OSM + TNF). Conditioned medium was collected at days 7, 11, and 17. (a) Aggrecanase-mediated aggrecan degradation was measured by the ³⁷⁴ARGSV-G2 enzyme-linked immunosorbent assay (ELISA), (b) matrix metalloproteinase (MMP)-mediated aggrecan degradation was quantified by the ³⁴²FFGVG-G2 ELISA, and (c) MMP-mediated collagen type II degradation was quantified in the CTX-II ELISA. **P < 0.01, ***P < 0.001. CTX-II, crosslinked C-terminal neo-epitopes of type II collagen.