Skip to main content

Advertisement

Table 1 Primers used for RT-PCR

From: Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells

Gene name Primer sequence Product length Annealing temperature Number of cycles
BMP-2 Forward: GCT CTT TCA ATG GAC GTG TC
Reverse: GCT CTG CTG AGG TGA TAA AC
514 bp 59°C 38
Chordin Forward: GAG AAC TTC AGG CCA ATG TC
Reverse: CAG TGG GTA TCC AAG GAA AG
654 bp 58°C 40
GAPDH Forward: CCA TCA CCA TCT TCC AGG AG
Reverse: CAT CCA CAG TCT TCT GGG TG
353 bp 60°C 32
  1. bp, base pairs; BMP, bone morphogenetic protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.