Table 1 Primers used for RT-PCR
From: Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells
Gene name | Primer sequence | Product length | Annealing temperature | Number of cycles |
|---|---|---|---|---|
BMP-2 | Forward: GCT CTT TCA ATG GAC GTG TC Reverse: GCT CTG CTG AGG TGA TAA AC | 514 bp | 59°C | 38 |
Chordin | Forward: GAG AAC TTC AGG CCA ATG TC Reverse: CAG TGG GTA TCC AAG GAA AG | 654 bp | 58°C | 40 |
GAPDH | Forward: CCA TCA CCA TCT TCC AGG AG Reverse: CAT CCA CAG TCT TCT GGG TG | 353 bp | 60°C | 32 |