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Table 1 Primers used for RT-PCR

From: Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells

Gene name Primer sequence Product length Annealing temperature Number of cycles
BMP-2 Forward: GCT CTT TCA ATG GAC GTG TC Reverse: GCT CTG CTG AGG TGA TAA AC 514 bp 59°C 38
Chordin Forward: GAG AAC TTC AGG CCA ATG TC Reverse: CAG TGG GTA TCC AAG GAA AG 654 bp 58°C 40
GAPDH Forward: CCA TCA CCA TCT TCC AGG AG Reverse: CAT CCA CAG TCT TCT GGG TG 353 bp 60°C 32
  1. bp, base pairs; BMP, bone morphogenetic protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.