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Figure 1 | Arthritis Research & Therapy

Figure 1

From: Therapeutic effects of antibodies to tumor necrosis factor-α, interleukin-6 and cytotoxic T-lymphocyte antigen 4 immunoglobulin in mice with glucose-6-phosphate isomerase induced arthritis

Figure 1

GPI-induced TNF-α and IFN-γ production from arthritic splenocytes in vitro. Spleens were removed from glucose-6-phosphate isomerase (GPI)-immunized DBA/1 mice (on day 8 after immunization), and then single-cell suspensions were prepared. MACS separated CD4+ T cells (1 × 106 cells/ml) were stimulated with 5 μg/ml GPI (or glutathione S-transferase [GST]) and antigen-presenting cells (APCs; 2 × 105 cells/ml, mitomycin treated) for 12 hours. The culture supernatants were collected and concentrations of tumor necrosis factor (TNF)-α, IFN-γ, IL-2, IL-4, IL-5, IL-6, IL-10, and IL-12p70 were measured by cytometric bead array. Data were averages of three independent experiments. Error bars represent ± standard error. *P < 0.05, by Mann-Whitney U-test.

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