Altered fractalkine cleavage in NOD salivary gland. Whole tissue lysates were prepared in phosphate-buffered saline (PBS) supplemented with protease inhibitor by mechanical homogenization followed by sonification. Lysates were then analysed by Western blotting. A representative picture of six individual experiments is shown. (a) Submandibular gland (SMGs) of 5-, 10- and 15-week-old nonobese diabetic (NOD) mice. (b) SMGs of 10-week-old (left) and 20-week-old (right) NOD, C57BL/6 and Balb/c mice. (c) Pancreas of 5- and 20-week-old NOD and Balb/c mice. Numbers represent mass (kDa), as determined using a molecular weight marker.