Figure 4

Effect of diacerein and rhein on the osteoclastic levels of (a) metalloprotease-13 (MMP-13) and (b) cathepsin K. Determination was performed in the conditioned medium for MMP-13 and on cell lysates for cathepsin K. Raw 264.7 cells were incubated for 5 days with RANKL (100 ng/mL), allowing the cells to differentiate into osteoclasts. After this period, the cells were incubated for 2 days together with RANKL in the presence or absence of interleukin-1-beta (IL-1β) (100 pg/mL) and diacerein or rhein (10 or 20 μg/mL). Data are expressed as fold changes compared with IL-1β-treated control, which was assigned a value of 1. Statistical analysis was performed versus IL-1β-treated control. RANKL, receptor activator of nuclear factor-κB ligand.