|
Time
|
Mice
|
IL-6 production (pg/ml)
|
Lipopolysaccharidea
|
|---|
| | |
0 μg/ml HMGB1
|
0.05 μg/ml HMGB1
|
0.5 μg/ml HMGB1
|
5 μg/ml HMGB1
| |
|---|
|
PHMGB1
| | | | | | |
|
24 hours
|
TNFα-/-
|
17.0 ± 2.3
|
17.6 ± 2.2
|
17.8 ± 2.4
|
13.1 ± 4.6
|
2,460 ± 226
|
| |
TNFα+/+
|
16.2 ± 1.9
|
15.9 ± 2.0
|
16.8 ± 3.2
|
7.3 ± 3.1
|
6,905 ± 1,763
|
|
48 hours
|
TNFα-/-
|
19.1 ± 3.0
|
20.1 ± 2.9
|
17.0 ± 1.6
|
26.3 ± 3.8
| |
| |
TNFα+/+
|
19.8 ± 2.2
|
18.3 ± 2.2
|
14.2 ± 2.3
|
20.2 ± 3.2
| |
|
RHMGB1
| | | | | | |
|
24 hours
|
TNFα-/-
|
28 ± 16
| |
304 ± 50* (314)
|
2,317 ± 264 (2,271)
|
146 ± 46* (130)
|
| |
TNFα+/+
|
68 ± 11
| |
602 ± 112 (558)
|
3,046 ± 757 (2,504)
|
344 ± 63 (359)
|
|
48 hours
|
TNFα-/-
|
213 ± 58
| |
468 ± 56* (500)
|
2,541 ± 258 (2,588)
|
238 ± 79 (207)
|
| |
TNFα+/+
|
263 ± 33
| |
1,133 ± 202 (1,062)
|
4,114 ± 941 (3,527)
|
438 ± 62 (408)
|
- Data presented as the mean ± standard error of the mean of 10 mice per each group (pHMGB1), and the mean ± standard error of the mean (median) of four mice in each group (rHMGB1). HMGB1, high mobility group box chromosomal protein 1. apHMGB1, 5 μg/ml; rHMGB1, 10 ng/ml. *P < 0.05 TNFα-/- mice versus control mice.
