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Table 1 Production of IL-6 following 24 hours and 48 hours of stimulation with different doses of purified recombinant endotoxin-free high mobility group box chromosomal protein 1 (pHMGB1) and mouse recombinant high mobility group box chromosomal protein 1 (rHMGB1)

From: Induction of arthritis by high mobility group box chromosomal protein 1 is independent of tumour necrosis factor signalling

Time Mice IL-6 production (pg/ml) Lipopolysaccharidea
   0 μg/ml HMGB1 0.05 μg/ml HMGB1 0.5 μg/ml HMGB1 5 μg/ml HMGB1  
PHMGB1       
   24 hours TNFα-/- 17.0 ± 2.3 17.6 ± 2.2 17.8 ± 2.4 13.1 ± 4.6 2,460 ± 226
  TNFα+/+ 16.2 ± 1.9 15.9 ± 2.0 16.8 ± 3.2 7.3 ± 3.1 6,905 ± 1,763
   48 hours TNFα-/- 19.1 ± 3.0 20.1 ± 2.9 17.0 ± 1.6 26.3 ± 3.8  
  TNFα+/+ 19.8 ± 2.2 18.3 ± 2.2 14.2 ± 2.3 20.2 ± 3.2  
RHMGB1       
   24 hours TNFα-/- 28 ± 16   304 ± 50* (314) 2,317 ± 264 (2,271) 146 ± 46* (130)
  TNFα+/+ 68 ± 11   602 ± 112 (558) 3,046 ± 757 (2,504) 344 ± 63 (359)
   48 hours TNFα-/- 213 ± 58   468 ± 56* (500) 2,541 ± 258 (2,588) 238 ± 79 (207)
  TNFα+/+ 263 ± 33   1,133 ± 202 (1,062) 4,114 ± 941 (3,527) 438 ± 62 (408)
  1. Data presented as the mean ± standard error of the mean of 10 mice per each group (pHMGB1), and the mean ± standard error of the mean (median) of four mice in each group (rHMGB1). HMGB1, high mobility group box chromosomal protein 1. apHMGB1, 5 μg/ml; rHMGB1, 10 ng/ml. *P < 0.05 TNFα-/- mice versus control mice.