Alternative splice variant mRNA expression. (a) RT-PCR detection of mRNA expression of FGFR4 (top panels) and FGFR4-ASV (bottom panels) across 20 normal tissues and nine cancers, including two cancer cell lines. The amplified RT-PCR products were separated on 1% agarose gels and visualized by ethidium bromide staining. bp, base pairs. (b) Expression profile heat map of the constitutively expressed (C) and matched splice variant (V) mRNAs. Transcripts were analyzed across 20 normal tissues and nine cancers, including two cancer cell lines. Amplification of the constitutive and splice variant sequences was performed using real-time PCR. Bar shows a color shift from green (high-level expression) to red (low-level expression), with the corresponding cycle threshold values indicated.