Table 1 Reverse transcription-polymerase chain reaction primers for differentiation-specific gene expression analysis
From: Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells
Genes | Primer sequences (5'-3') | Position, base pairs | Predicted size, base pairs |
|---|---|---|---|
Housekeeping gene | Â | Â | Â |
   GAPDH | Sense: GGACTCATGACCACAGTCCATGCC | 619–770 | 152 |
| Â | Antisense: TCAGGGATGACCTTGCCCACA | Â | Â |
Bone-specific genes | Â | Â | Â |
   ALP | Sense: TGGAGCTTCAGAAGCTCAACACCA | 379–832 | 454 |
| Â | Antisense: ATCTCGTTGTCTGAGTACCAGTCC | Â | Â |
   OC | Sense: ATGAGAGCCCTCACACTCCTC | 19–312 | 294 |
| Â | Antisense: GCCGTAGAAGCGCCGATAGGC | Â | Â |
Adipose-specific genes | Â | Â | Â |
   LPL | Sense: GAGATTTCTCTGTATGGCACC | 1,457–1,732 | 276 |
| Â | Antisense: CTGCAAATGAGACACTTTCTC | Â | Â |
   PPARγ | Sense: TGAATGTGAAGCCCATTGAA | 1,476–1,636 | 161 |
| Â | Antisense: CTGCAGTAGCTGCACGTGTT | Â | Â |
Cartilage-specific genes | Â | Â | Â |
   AGN | Sense: TGCGGGTCAACAGTGCCTATC | 655–836 | 182 |
| Â | Antisense: CACGATGCCTTTCACCACGAC | Â | Â |
   COL2A1 | Sense: GGAAACTTTGCTGCCCAGATG | 710–876 | 167 |
| Â | Antisense: TCACCAGGTTCACCAGGATTGC | Â | Â |