Ex vivo sequential analysis of cell attachment to rabbit cartilage defects by local adherent technique. (a) Scheme for the method: image a, cartilage defect of the femoral condyle was faced upward and the defect was filled with 106 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate-labeled rabbit synovial mesenchymal stem cells in 100 μl PBS; image b, defect was held stationary for 2.5, 5, 7.5, 10, and 15 minutes; image c, femur was turned with the defect side down for 10 minutes so that nonadhered cells in the defect fell in the culture medium. The nonattached cell number was then determined, and the attached cell number was extrapolated. (b) Cell number attached to the cartilage defects by the local adherent technique. Data expressed as the mean ± standard deviation (n = 3).