Molecular and morphological events during a 10-minute period. (a) Intercellular adhesion molecule 1 (ICAM-1) expression in human synovial mesenchymal stem cells (MSCs) 1 minute and 10 minutes after plating on glass slides. ICAM-1-positive cells are shown as light shading, and nuclei as dark shading. Bars = 100 μm. (b) ICAM-1-positive cell rate. The number of ICAM-1-positive cells and nuclei were counted in three high-power fields. Data expressed as the mean ± standard deviation (*P < 0.05 by Mann–Whitney U test). (c) Morphological alterations of human synovial MSCs between 1 minute and 10 minutes after plating on a culture dish. Bars = 50 μm.