|
Target
|
Forward primer 5' to 3'
|
Reverse primer 5' to 3'
|
Amplicon size, base pairs (bp)
|
|
18S
|
GCC ATG CAT GTC TAA GTA CG
|
GCT GGC ACC AGA CTT GCC
|
574 bp
|
|
Caveolin-1
|
AAG GAG ATC GAC CTG G
|
GGA ATA GAC ACG GCT G
|
309 bp
|
|
Real-time PCR primers and probes
|
|
Target
|
Forward primer 5' to 3'
|
Probe 5' to 3'
|
Reverse primer 5' to 3'
|
|
18S
|
PDAR
|
PDAR (VIC-TAMRA)
|
PDAR
|
|
Caveolin-1
|
ACT TGC AAC CGT CTG TTA TGC T
|
FAM – ACA TGG CCC CTC CCC – MGB
|
GCA AAG GGA TGC TTG GAT TAG GT
|
|
p16INK4a
|
GGC TCT ACA CAA GCT TCC TTT CC
|
FAM – ACC CTG GCT CTG ACC A – MGB
|
TCA TGA CCT GCC AGA GAG AAC A
|
