Skip to main content

Table 1 Details of polymerase chain reaction (PCR) primers, probes, and amplicon sizes

From: Caveolin-1 expression and stress-induced premature senescence in human intervertebral disc degeneration

Conventional PCR conditions
Target Forward primer 5' to 3' Reverse primer 5' to 3' Amplicon size, base pairs (bp)
18S GCC ATG CAT GTC TAA GTA CG GCT GGC ACC AGA CTT GCC 574 bp
Caveolin-1 AAG GAG ATC GAC CTG G GGA ATA GAC ACG GCT G 309 bp
Real-time PCR primers and probes
Target Forward primer 5' to 3' Probe 5' to 3' Reverse primer 5' to 3'
18S PDAR PDAR (VIC-TAMRA) PDAR
Caveolin-1 ACT TGC AAC CGT CTG TTA TGC T FAM – ACA TGG CCC CTC CCC – MGB GCA AAG GGA TGC TTG GAT TAG GT
p16INK4a GGC TCT ACA CAA GCT TCC TTT CC FAM – ACC CTG GCT CTG ACC A – MGB TCA TGA CCT GCC AGA GAG AAC A
  1. PDAR, pre-developed assay reagent.