Conventional PCR conditions | |||
---|---|---|---|
Target | Forward primer 5' to 3' | Reverse primer 5' to 3' | Amplicon size, base pairs (bp) |
18S | GCC ATG CAT GTC TAA GTA CG | GCT GGC ACC AGA CTT GCC | 574 bp |
Caveolin-1 | AAG GAG ATC GAC CTG G | GGA ATA GAC ACG GCT G | 309 bp |
Real-time PCR primers and probes | |||
Target | Forward primer 5' to 3' | Probe 5' to 3' | Reverse primer 5' to 3' |
18S | PDAR | PDAR (VIC-TAMRA) | PDAR |
Caveolin-1 | ACT TGC AAC CGT CTG TTA TGC T | FAM – ACA TGG CCC CTC CCC – MGB | GCA AAG GGA TGC TTG GAT TAG GT |
p16INK4a | GGC TCT ACA CAA GCT TCC TTT CC | FAM – ACC CTG GCT CTG ACC A – MGB | TCA TGA CCT GCC AGA GAG AAC A |