Figure 1From: 4-Hydroxynonenal induces apoptosis in human osteoarthritic chondrocytes: the protective role of glutathione-S-transferase4-Hydroxynonenal (HNE)-induced cell death. (a) Chondrocytes were pre-incubated for 1 hour with or without 200 μM N-acetyl-cysteine (NAC) followed by another incubation for 16 hours with increasing concentrations of HNE (0 to 30 μM). Chondrocytes were pre-incubated for 1 hour with or without (b) 20 μg/mL anti-Fas/CD95 antibody or (c) 50 and 100 μM INH2BP followed by another incubation for 16 hours with or without 30 μM HNE. Cell viability was evaluated by MTT assay. Data are mean ± standard error of the mean (n = 8). Statistics: Student unpaired t test; ***P < 0.001 (30 μM HNE versus untreated cells), #P < 0.05 (30 μM HNE+inhibitor versus 30 μM HNE).Back to article page