Figure 4
From: Decreased levels of the gelsolin plasma isoform in patients with rheumatoid arthritis
Actin and gelsolin (GSN)-actin complexes in synovial fluid (SF). (a) Representative immunoblot showing actin in SFs of rheumatoid arthritis (RA) patients. The beta isoform of actin is present to various degrees in SF from RA patients. (b) Representative immunoblot showing results from immunoprecipitation of GSN-actin complexes using the 2c4 anti-GSN antibody. SF number 23 (S23) was incubated with 2c4 or control IgG. Note that, when the band densities for S23 incubated with 2c4 versus control IgG are compared, the 2c4 antibody is seen to pull down more actin than the control IgG, indicating that actin is bound to GSN in SF.