The cytokine production from spleen cells in response to denatured type II collagen (CII) and ConA in RP105-deficient mice. Mice were immunized with CII with complete Freund's adjuvant on day 0 and with CII with incomplete Freund's adjuvant on day 21. The spleen cells from day-28 mice were harvested and stimulated with CII or ConA (5 μg/mL) for 48 hours. Interferon-gamma (IFN-γ) (a), tumor necrosis factor-alpha (TNF-α) (b), interleukin-4 (IL-4) (c), and interleukin-2 (IL-2) (d) produced in the culture supernatant were measured by cytometric beads assay. The results from two experiments (eight mice per experiment) are shown and expressed as the mean ± standard error (*P < 0.05, **P < 0.01, Mann-Whitney U test).