Figure 5
From: Arthritogenic T cell epitope in glucose-6-phosphate isomerase-induced arthritis
Cross-reactivity with peptides derived from mouse glucose-6-phosphate isomerase (GPI). (a) Draining lymph node (DLN) cells taken from hGPI325–339-immunised mice on day 5 were cultured with 10 μM of hGPI325–339, mGPI325–339, hGPI544–558 or mGPI544–558 for 24 hours. The supernatants were assayed for interleukin (IL) 17 by ELISA. Data are averages ± standard deviation of three culture-wells. Representative data of three independent experiments. (b) DLN cells taken from hGPI325–339- or hGPI544–558-immunised mice on day 5 were cultured with 10 μM of hGPI325–339 and mGPI325–339 or hGPI544–558 and mGPI544–558, respectively. GoldiStop was added at the last four hours of each culture. Flow cytometry for IL-17 and interferon (IFN) γ was gated in CD3+, CD4high cells. Representative flow cytometry data of three independent experiments with two mice per experiment.