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Table 4 Agreement between different methods in a cohort of 100 patients with systemic lupus erythematosus

From: Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies

 

ELISA (>1.5 RU) versus ALBIA (>350 LU)

 
 

ELISA +

ELISA -

 

ALBIA +

8

3

11

ALBIA -

21

68

89

 

29

71

 
 

ALBIA (>350 LU) versus LIA

 
 

LIA +

LIA -

 

ALBIA +

5

6

11

ALBIA -

5

84

89

 

10

90

 
 

ELISA (>1.5 RU) versus LIA

 
 

ELISA +

ELISA -

 

LIA +

10

0

10

LIA -

19

71

90

 

29

71

 
 

ELISA (>1.5 RU) versus IIF (CSP)

 
 

ELISA +

ELISA -

 

CSP +

7

4

11

CSP -

22

67

89

 

29

71

 
 

ALBIA (>350 LU) versus IIF (CSP)

 
 

ALBIA +

ALBIA -

 

CSP +

3

8

11

CSP -

8

81

89

 

11

89

 
 

LIA versus IIF (CSP)

 
 

LIA +

LIA -

 

CSP +

4

7

11

CSP -

6

83

89

 

10

90

 

Kappa agreements

 

ALBIA (350 LU)

LIA

IIF

ELISA

0.29

0.43

0.23

LIA

0.38

-

-

  1. ALBIA, addressable laser bead immunoassay; CSP, cytoplasmic staining pattern; ELISA, enzyme-linked immunosorbent assay; IIF, indirect immunofluorescence; LIA, line immunoassay; LU, luminescence units; RU, relative units.