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Table 4 Agreement between different methods in a cohort of 100 patients with systemic lupus erythematosus

From: Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies

  ELISA (>1.5 RU) versus ALBIA (>350 LU)  
  ELISA + ELISA -  
ALBIA + 8 3 11
ALBIA - 21 68 89
  29 71  
  ALBIA (>350 LU) versus LIA  
  LIA + LIA -  
ALBIA + 5 6 11
ALBIA - 5 84 89
  10 90  
  ELISA (>1.5 RU) versus LIA  
  ELISA + ELISA -  
LIA + 10 0 10
LIA - 19 71 90
  29 71  
  ELISA (>1.5 RU) versus IIF (CSP)  
  ELISA + ELISA -  
CSP + 7 4 11
CSP - 22 67 89
  29 71  
  ALBIA (>350 LU) versus IIF (CSP)  
  ALBIA + ALBIA -  
CSP + 3 8 11
CSP - 8 81 89
  11 89  
  LIA versus IIF (CSP)  
  LIA + LIA -  
CSP + 4 7 11
CSP - 6 83 89
  10 90  
Kappa agreements
  ALBIA (350 LU) LIA IIF
ELISA 0.29 0.43 0.23
LIA 0.38 - -
  1. ALBIA, addressable laser bead immunoassay; CSP, cytoplasmic staining pattern; ELISA, enzyme-linked immunosorbent assay; IIF, indirect immunofluorescence; LIA, line immunoassay; LU, luminescence units; RU, relative units.
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