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Figure 4 | Arthritis Research & Therapy

Figure 4

From: Collagen-specific T-cell repertoire in blood and synovial fluid varies with disease activity in early rheumatoid arthritis

Figure 4

CD4+CD25+ cell depletion from PBMCs does not restore proliferation in vitro of the original TCR repertoire. (a, b) Dual colour dot plot displaying the co-expression of CD4 and CD25 in the lymphocyte population. The regions used to measure the proportion of CD4+CD25high (Treg cells) are shown. (Panel a) Treg cells are 1.33% and 2.9% in the total lymphocyte population and within the CD4+ subset, respectively. (Panel b) Treg cells are efficiently removed (0.05% and 1.1% in the total lymphocyte population and within the CD4+ subset, respectively) after depletion with anti-CD25 immunomagnetic microbeads. (c) Effect of Treg cell removal and of excess Treg cells on T-cell proliferative response. Bars show the proliferation index measured by ModFit™/Cell Proliferation Model™ software, as detailed in the Materials and methods section. (d) RSI of the TCR repertoire involved in the response to huCollp261–273 when examined in the unseparated PBMCs (see panel a). Dashed bars indicate the RSI of the BV11-BJ2.2 (138 bases) rearrangement. (e) RSI of the TCR repertoire involved in the response to huCollp261–273 when examined in the CD4+CD25+-depleted PBMC. huCollp261–273, human collagen peptide 261–273; PBMC, peripheral blood mononuclear cell; RSI, rate stimulation index; TCR, T-cell receptor.

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