Figure 5
Effects of T-614 on serum IgG levels in CIA rats. Serum was collected on day 36. Anti-CII (total IgG, IgM, IgG1, IgG2a, and IgG2b) levels increased as disease progressed. Combination therapy reduced the total anti-CII antibody level significantly, as well as levels of IgM, IgG1, IgG2a, and IgG2b. High dose T-614(20 mg/kg per day) also decreased levels of total IgG, IgM and IgG2a, whereas low-dose of T-614 (5 mg/kg per day) or MTX (1 mg/kg every 3 days) had an effect only on IgG2a level. Data are expressed as mean ± standard error of the mean (n = 5 to 7). *P < 0.05, versus the vehicle-treated rats.