Differences in temporal loss of secretory function in various C57BL/6.NOD-Aec1Aec2R(n) mice. Male and female sibling mice of parental C57BL/6.NOD-Aec1Aec2 (P-DC) and C57BL/6.NOD-Aec1Aec2R(n) mice were injected with isoproterenol/pilocarpine, first at 8 weeks of age and then at 20 or 24 weeks of age, to stimulate saliva secretion. Saliva was collected from each mouse for 10 minutes starting 1 minute after injection of the secretagogue. The volume of each sample was measured and standardized relative to the weight of the mouse. Temporal reductions in saliva secretions, a marker for onset of clinical disease, were used to identify genetic regions containing genes necessary for development of salivary gland dysfunction and Sjögren syndrome. NS, not significant; RI, recombinant inbred.