Figure 3

Combination treatment with LG268 and rosiglitazone results in increased inhibition of matrix metalloproteinase-1 (MMP-1) and MMP-13 expression. SW-1353 cells were treated for 24 hours with 50 nM LG268, 50 nM rosiglitazone, or both compounds followed by 24 hours of treatment with 1 ng/mL interleukin-1-beta (IL-1β). Total RNA was harvested, and MMP (a) mRNA and (b) heterogeneous nuclear RNA levels were quantified using real-time reverse transcription-polymerase chain reaction. Y values are given as molecules of MMP per molecule of GAPDH (glyceraldehyde 3-phosphate dehydrogenase). P values above each vertical bar were determined for the difference from the IL-1β sample, and P values above the horizontal bars were determined for the difference between samples on either end of the bar. In all cases, P values were calculated using the Student t test (**P < 0.005, ***P < 0.0005). 268, LG100268; NoTx, no treatment; Rosi, rosiglitazone.