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Figure 1 | Arthritis Research & Therapy

Figure 1

From: Synergistic role of c-Myc and ERK1/2 in the mitogenic response to TGFβ-1 in cultured rat nucleus pulposus cells

Figure 1

Effect of transforming growth factor β1 (TGFβ1) treatment on mRNA expression in different cell types (a), Cells were treated with or without 5 ng/mL TGFβ1 for 24 h. The expression of c-myc in nucleus pulposus cells (NP), in articular chondrocytes (AC) and keratinocytes (KT) are presented. The expression of p15, p21 and p27 in NP was also determined. Time course of c-myc expression in NP treated with 5 ng/mL TGFβ1 (b). The graph shows the relative intensities of c-myc bands normalized for β-actin levels by densitographic analysis. Incubation for 24 h with medium containing various concentrations of fetal bovine serum (FBS) did not alter the level of c-myc expression in NP (c). The reverse transcription-polymerase chain reaction (RT-PCR) was performed on total RNA extracted from the cells. β-actin was used as an internal control.

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