Figure 3

c-Myc transcription inhibition prevents transforming growth factor β1 (TGFβ1)-stimulated cell proliferation. Serum-deprived cells in 96-well plates were treated with 5 or 20 ng/mL TGFβ1 (abbreviated to T) with or without 8, 12, 16 μM 10058-F4. Cell proliferation was evaluated by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay on day 3 after treatment. Five replicates per experimental condition were made. Data are normalized to values obtained for untreated cells cultured in 0.5% serum containing medium and represented as mean ± standard error of the mean (SEM) (**p < 0.01 when compared with control, #p < 0.01 when compared with the TGFβ1-treated group).