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Figure 3 | Arthritis Research & Therapy

Figure 3

From: All-trans retinoic acid suppresses interleukin-6 expression in interleukin-1-stimulated synovial fibroblasts by inhibition of ERK1/2pathway independently of RAR activation

Figure 3

Effect of selective retinoic acid receptor (RAR) agonists on interleukin-1 (IL-1)-induced expression of IL-6. (a) To control the ability of the selective agonists to trigger RAR-dependent responses, rat synovial fibroblasts were stimulated for 6 hours with 0.1 μM of RAR agonist (BMS-753 for RAR-α, BMS-453 for RAR-β, or BMS-961 for RAR-γ), and the mRNA level of the target gene RAR-β normalized to ribosomal protein S29 (RP29) was studied by real-time polymerase chain reaction (PCR). (b) The suppressive effect of RAR-selective agonists on the IL-6 mRNA level normalized to RP29 was studied by real-time PCR in cells stimulated with 10 ng/mL of IL-1β in the presence or absence of 0.1 or 1 μM of RAR agonist. Data are expressed as mean ± standard deviation of values from at least three independent experiments. Statistically significant differences from the control are indicated as *P < 0.05.

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Arthritis Research & Therapy

ISSN: 1478-6362

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