Figure 2
From: Hypoxia. HIF-mediated articular chondrocyte function: prospects for cartilage repair
Hypoxia enhances chondrogenic differentiation of mesenchymal stem cells (MSCs). Clonally derived MSCs were isolated from the bone marrow of 5-week-old mice. After 1 week in chondrogenic medium (containing 5 ng/ml transforming growth factor-β3) at different oxygen concentrations, cultures were stained for cartilage-specific type II collagen. Enhanced collagen II staining was observed in cultures exposed to reduced oxygen concentrations.