Figure 5

Effect of cytokines and dexamethasone on FcγRI/CD64 expression in vitro. (a) Direct effects of cytokines and C-reactive protein (CRP) on FcγRI/CD64 expression on circulating monocytes. Peripheral blood mononuclear cells from healthy subjects were cultured with recombinant IFNα (4 ng/ml), IFNγ (2 ng/ml), IL-4 (4 ng/ml), IL-6 (4 ng/ml), IL-8 (4 ng/ml), IL-12 (4 ng/ml) or CRP (50 ng/ml) for 19 hours in vitro. FcγRI/CD64 expression (mean fluorescence intensity (MFI)) was analyzed by flow cytometry. Values represent the mean ± standard error of the mean (SEM) from five independent experiments. *P < 0.001 compared with medium alone. (b) Effect of dexamethasone on monocyte FcγRI/CD64 expression in vitro. Values represent the mean ± SEM from three independent experiments. (c) Effect of dexamethasone on the upregulation of FcγRI/CD64 by IFNα, IFNγ, and IL-12. Values represent the mean ± SEM from two independent experiments.