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Figure 4 | Arthritis Research & Therapy

Figure 4

From: The response to oestrogen deprivation of the cartilage collagen degradation marker, CTX-II, is unique compared with other markers of collagen turnover

Figure 4

Illustrative examples of immunostainigs of CTX-II and PIIANP and in situ hybridisations of type IIA collagen mRNA. All immunohistochemistry sections were stained with DAB+ (brown) and counterstained with Mayers acidic haematoxylin (blue). In situ hybridisations were developed with silver grains (black) and counterstained with H&E staining. CTX-II immunoreactivity was observed (a) around chondrocytes at the inner zone, (b) at superficial matrix of the upper zone, (c) around the round and flat chondrocytes of the upper and deep zone and (d) in the growth plate. PIIANP immunoreactivity was observed (e) around and within the lacunas of the inner zone, (f) in the superficial matrix of a section and (g) in the proliferating cells of the growth plate. (h) Preimmune serum control for CTX-II at the growth plate (PIIANP preimmune showed similar results, data not shown). Col IIA mRNA expression was observed (i) in the inner zone, (j) in the middle of a section showing the upper and deep zone, (k) in the proliferating chondrocytes of the growth plate. (l) Negative control using Col IIA sense probe. All sections were captured at ×20 magnification.

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