Dissection of a locus on mouse chromosome 5 reveals arthritis promoting and inhibitory genes

Table 1 Incidence and mean maximum score of collagen-induced arthritis (CIA) in Eae39 congenic mice^a

Phenotype	Marker	Mbp^b	Group	a/a^c	a/b	b/b	p-value^d
Incidence	D5Mit113	77.68	Total	10/14 (71%)	7/12 (58%)	8/42 (19%)	0.0005
			Males	3/5 (60%)	4/9 (44%)	2/21 (10%)	0.0222
			Females	7/9 (78%)	3/3 (100%)	6/21 (29%)	0.0082
	D5Mit136	119.18	Total	11/15 (73%)	3/19 (16%)	11/34 (32%)	0.0019
			Males	3/5 (60%)	0/10 (0%)	6/20 (30%)	0.0345
			Females	8/10 (80%)	3/9 (33%)	5/14 (36%)	0.0573
Mean max score^e	D5Mit113	77.68	Total	23 ± 5	21 ± 5	21 ± 7	0.7766
			Males	31 ± 15	20 ± 10	14 ± 2	0.3280
			Females	20 ± 5	22 ± 10	24 ± 9	0.9604
	D5Mit136	119.18	Total	21 ± 5	6 ± 4	27 ± 15	0.1034
			Males	31 ± 15		18 ± 3	0.1948
			Females	18 ± 5	6 ± 4	37 ± 6	0.0317

^aShows the mean incidence and the mean maximum score of mice with the respective genotypes on markers D5Mit113 and D5Mit136. Calculations were made on all mice in Figures 1 and 3 (a to l), and littermate controls. The sub-interval congenic mice were generated by intercrossing the C1 congenic mice (Figure 1).
^bMbp = mega base pairs. The Mbp position is according to Ensembl release 49.
^ca/a = homozygous RIIIS/J alleles; b/b = homozygous B10.RIII alleles; a/b = heterozygous.
^dStatistics for incidence was calculated with Chi squared test. Statistics for severity was calculated with Kruskal-Wallis test and Mann-Whitney U test.
^eMean of the maximum score for all affected mice in Figures 1 and 3.

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